In vitro deletional mutagenesis for bacterial production of the 20,000-dalton form of human pituitary growth hormone.
Adelman JP, Hayflick JS, Vasser M, Seeburg PH.Links to PubMed are also available for Selected References. Get a printable copy (PDF file) of the complete article (3.1M), or click on a page image below to browse page by page. Full textįull text is available as a scanned copy of the original print version.
Both receptors expressed in cultured mammalian cells bind spiperone with high affinity and inhibit adenylyl cyclase, as expected of the D2 receptor subtype. Hence, the two receptor forms are generated by differential splicing possibly to permit coupling to different G proteins. Analysis of the human gene showed that the additional peptide sequence is encoded by a separate exon. The larger form was predominant in these tissues and, as shown in the rat, expressed by dopaminergic and dopaminoceptive neurons. Results from polymerase chain reactions as well as in situ hybridization revealed that mRNA encoding both receptor forms is present in pituitary and brain of both rat and man.
Cloned human dopamine D2 receptor cDNA was isolated from a pituitary cDNA library and found to encode an additional 29 amino acid residues in the predicted intracellular domain between transmembrane regions 5 and 6 relative to a previously described rat brain D2 receptor.
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